Relapsing Fever Spirochete in Seabird Tick, Japan
نویسندگان
چکیده
Phylogenetic analysis based on A) the 16S ribosomal RNA gene (16SrRNA) and B) flagellin gene of Borrelia spp. Representative Borrelia spp. were used. GenBank accession numbers are in parentheses. For the amplification and direct sequencing of 16SrRNA, the 4 PCR primers rrs-F1 (5′-ATAACGAAGAGTTTGATCCTGGCT-3′), rrs-F2 (5′-GGTGTAAGGGTGGAATCTGTTG-3′), rrs-R3 (5′-TTTCGTGACTCAGCGTCAGT-3′), and rrs-R4 (5′-AAAGGAGGTGATCCAGCCRCACT-3′) were used. These primers were designated by DNA sequence alignment of 5 of 16SrRNA (GenBank accession nos.
منابع مشابه
Bloodstream- versus tick-associated variants of a relapsing fever bacterium.
The relapsing fever spirochete, Borrelia hermsii, alternates infections between a mammal and a tick vector. Whether the spirochete changes phenotypically in the different hosts was examined by allowing the tick vector Ornithodoros hermsi to feed on mice infected with serotype 7 or serotype 8 of B. hermsii. Upon infection of ticks, the spirochetal serotype-specific variable major proteins (Vmps)...
متن کاملTick-borne Relapsing Fever and Borrelia hermsii, Los Angeles County, California, USA
The primary cause of tick-borne relapsing fever in western North America is Borrelia hermsii, a rodent-associated spirochete transmitted by the fast-feeding soft tick Ornithodoros hermsi. We describe a patient who had an illness consistent with relapsing fever after exposure in the mountains near Los Angeles, California, USA. The patient's convalescent-phase serum was seropositive for B. hermsi...
متن کاملTick Surveillance for Relapsing Fever Spirochete Borrelia miyamotoi in Hokkaido, Japan
During 2012-2013, a total of 4325 host-seeking adult ticks belonging to the genus Ixodes were collected from various localities of Hokkaido, the northernmost island of Japan. Tick lysates were subjected to real-time PCR assay to detect borrelial infection. The assay was designed for specific detection of the Relapsing fever spirochete Borrelia miyamotoi and for unspecific detection of Lyme dise...
متن کاملTick-borne relapsing fever.
Tick-borne relapsing fever is characterized by recurring fevers separated by afebrile periods and is accompanied by nonspecific constitutional symptoms. It occurs after a patient has been bitten by a tick infected with a Borrelia spirochete. The diagnosis of tick-borne relapsing fever requires an accurate characterization of the fever and a thorough medical, social, and travel history of the pa...
متن کاملEcological niche modeling and distribution of Ornithodoros hermsi associated with tick-borne relapsing fever in western North America
Tick-borne relapsing fever in western North America is a zoonosis caused by the spirochete bacterium, Borrelia hermsii, which is transmitted by the bite of infected Ornithodoros hermsi ticks. The pathogen is maintained in natural cycles involving small rodent hosts such as chipmunks and tree squirrels, as well as the tick vector. In order for these ticks to establish sustained and viable popula...
متن کامل